Skeletal myogenic differentiation of human periodontal ligament stromal cells isolated from orthodontically extracted premolars
송민정, 김하나, 최윤정, 김경호, 정주령,
소속 상세정보
송민정 ( Song Min-Jung ) - Gangnam Severance Dental Hospital Department of Orthodontics
김하나 ( Kim Ha-Na ) - Gangnam Severance Dental Hospital Department of Orthodontics
최윤정 ( Choi Yoon-Jeong ) - Gangnam Severance Dental Hospital Department of Orthodontics
김경호 ( Kim Kyung-Ho ) - Gangnam Severance Dental Hospital Department of Orthodontics
정주령 ( Chung Choo-Ryung ) - Gangnam Severance Dental Hospital Department of Orthodontics
KMID : 0361920120420050249
Abstract
Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation.
Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2’-deoxycytidine (5-Aza) for DNA demethylation.
Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment.
Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.
키워드
Human periodontal ligament stem cell;Myogenic differentiation;Cell biology;Molecular biology;Soft tissue
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